Free Biotechnology MCQs with Answers

• A. Gene therapy
• B. Chemotherapy
• C. Immunotherapy
• D. Radiation therapy

Explanation: Gene therapy is a technique of genetic engineering which involves the replacement of a faulty/disease-causing gene by a normal healthy…

• A. Ability to incorporate cloned DNA
• B. Ability to replicate independently of chromosomal DNA
• C. Ability to be isolated from the host's genomic DNA
• D. All of the above

Explanation: Plasmids are critical tools in molecular biology due to their ability to incorporate foreign DNA, replicate independently, and be easily…

• A. Two different sources
• B. A single source
• C. Two identical sources
• D. Three identical sources

Explanation: Recombinant DNA technology is the process of joining together DNA from two different species, resulting in a new genetic combination that…

• A. Pneumococcus
• B. E. Coli
• C. Rhizobium
• D. Nostoc

Explanation: E.coli is correct. E. coli has been programmed to make human growth hormones for the treatment of growth deficiencies and insulin for…

• A. Plastids
• B. Plasmid
• C. Nucleoid
• D. Pili

Explanation: A plasmid is a small fragment of extra genetic material, double-stranded DNA.

• A. Transcriptase
• B. Reverse transcriptase
• C. Oxidoreductase
• D. Ligases

Explanation: Reverse transcriptase is responsible for conversion of single stranded RNA into double stranded DNA. Hence this option is correct.

• A. DNA fingerprinting
• B. Fingerprinting
• C. Handwriting analysis
• D. Voiceprint analysis

Explanation: This is the correct answer. DNA fingerprinting is a forensic technique used to identify individuals based on their unique DNA profiles.

• A. Sterlization
• B. Filtration
• C. Desiccation
• D. Radiation

Explanation: Autoclave chambers, also known as autoclaves or steam sterilizers, are used to sterilize surgical instruments and other medical equipment…

• A. Radish
• B. Corn
• C. Maize
• D. Carrot
• E. Mango

Explanation: The correct answer is Carrot. F.C Steward was a pioneer in plant tissue culture and used carrot phloem tissue to show that plant cells…

• A. Under the influence of agar
• B. By diffusion
• C. Under the influence of an electric field
• D. Active transport
• E. By osmosis

Explanation: In gel electrophoresis, molecules migrate through the gel under the influence of an electric field.

• A. New males
• B. New females
• C. New breeds
• D. New organs

Explanation: Correct. Artificial selection leads to the development of new breeds of plants and animals with improved or specialized characteristics…

• A. Rapid growth
• B. Rapid aging
• C. Identical offspring
• D. Absence of mitosis

Explanation: Cloned animals tend to age faster due to shortened telomeres, inherited from the donor cell.

• A. Stem
• B. Root
• C. Flower
• D. Leaf

Explanation: Meloidogyne incognita, commonly known as the root-knot nematode, infects the roots of various plant species, including tobacco.

• A. Omega-3
• B. Vitamin A
• C. Vitamin B
• D. Vitamin C

Explanation: Golden rice is a transgenic variety of rice (Oryza sativa) which contains good quantities of B­-carotene (provitamin A - inactive state of…

• A. Exposing the plants to cold for a brief period
• B. Allowing the plant roots to stand in water
• C. Infection of the plant by Agrobacterium tumefaciens
• D. Altering the pH of the soil and then heat­ shocking the plants.

Explanation: Ti plasmid (tumor inducing) from the soil bacterium Agrobacterium tumefaciens is effectively used as a vector for gene transfer to plant…

• A. Action of gut microorganisms
• B. Presence of conversion factors in the insect gut
• C. Alkaline pH of the insect gut
• D. Acidic pH of the insect gut

Explanation: Soil bacterium Bacillus thuringiensis produces proteins that kill certain insects like lepidopterans (tobacco budworm, armyworm)…

• A. Insects
• B. Herbicides
• C. Fungi
• D. Bacteria

Explanation: Glyphosate is a broad-spectrum herbicide that especially kills broad-leaved herbs.

• A. Insulin
• B. Estrogen
• C. Thyroxin
• D. Progesterone

Explanation: The recombinant DNA technological processes have made a great impact in the area of health care by mass production of safe and more…

• A. Synthesis of complementary DNA and RNA using reverse transcriptase
• B. Silencing of specific mRNA due to complementary RNA
• C. Interference of RNA in the synthesis of DNA
• D. Synthesis of mRNA from DNA

Explanation: RNA interference (RNAi) is the phenomenon of inhibiting the activity of a gene through the production of both sense and antisense RNA.

• A. Adenovirus
• B. Cosmid
• C. Ri plasmid
• D. Ti plasmid

Explanation: Gene therapy is a corrective therapy that is given to patients with diseases caused by some gene defects.