Free Biotechnology MCQs with Answers
731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.
Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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Read the Biotechnology notesFree MDCAT chapter notes with key terms731 questions · page 23 of 37
- A. DNA Polymerase I
- B. Taq Polymerase
- C. DNA Polymerase III
- D. RNA Polymerase
Explanation: Taq polymerase is an enzyme used in DNA synthesis and proves to be most active at 70 to 80 degree Celsius, and can function even near 100…
Correct answer: Taq Polymerase- A. Hypercholesterolemia
- B. Coronary Artery Angioplasty
- C. Cystic Fibrosis
- D. Severe Combined Immunodeficiency Syndrome (SCID)
Explanation: Gene therapy using liposomes is used to treat cystic fibrosis. Liposomes are microscopic vesicles of lipoproteins, used to deliver the…
Correct answer: Cystic Fibrosis- A. Hypercholesterolemia
- B. Severe Combined Immunodeficiency Syndrome (SCID)
- C. Cystic Fibrosis
- D. Coronary Artery Angioplasty
Explanation: Gene therapy for SCID involves the removal of bone marrow stem cells from the patient.
Correct answer: Severe Combined Immunodeficiency Syndrome (SCID)- A. DNA ligase
- B. Primer
- C. Heat
- D. Helicase
Explanation: In PCR (polymerase chain reaction), the two strands of DNA are separated by raising the temperature.
Correct answer: Heat- A. Lambda phage
- B. Gamma phage
- C. T2 phage
- D. T4 phage
Explanation: Modified Lambda phages are used as a vector to introduce foreign DNA into a cell due to their efficiency at packaging and injecting DNA…
Correct answer: Lambda phage- A. Viruses
- B. Bacteria
- C. Fungi
- D. Plants
Explanation: Restriction endonucleases cleave into fragments near or at recognition sites.
Correct answer: Bacteria- A. pBR 101
- B. EcoR1
- C. EcoR3
- D. Both pBR 101 and EcoR3
Explanation: EcoRI is a restriction of endonuclease enzymes isolated from species. It is a restriction enzyme that cleaves DNA double helices into…
Correct answer: EcoR1- A. RNA
- B. EcoR1
- C. Template RNA
- D. Template DNA
Explanation: The main function of DNA polymerase is to synthesize DNA from deoxyribonucleotides, the building blocks of DNA.
Correct answer: Template DNA- A. Fishelsoni
- B. Thermus aquaticus
- C. E. Coli
- D. Both Fishelsoni and Thermus aquaticus
Explanation: Thermus aquaticus is a bacterium that lives in hot springs, and hydrothermal vents, and Tqa polymerase was identified as an enzyme able to…
Correct answer: Thermus aquaticus- A. PCR
- B. Tissue Culture
- C. Micropropagation
- D. Macropropagation
Explanation: Micropropagation is the artificial process of producing plants vegetatively through tissue culture or cell culture techniques.
Correct answer: Micropropagation- A. PCR
- B. PBr 103
- C. Eco R1
- D. Reverse transcriptase
Explanation: The general role of reverse transcriptase is to convert RNA sequences to cDNA sequences that are capable of inserting into different areas…
Correct answer: Reverse transcriptase- A. BAC
- B. YAC
- C. Cosmids
- D. EcoRI
Explanation: Cloning: It is a technique for developing a large number of genes, identical cells, tissues, or organisms.
Correct answer: EcoRI- A. 2 different sources
- B. Single source
- C. 2 same sources
- D. 3 same sources
Explanation: Recombinant DNA technology is the joining together of DNA molecules from two different species.
Correct answer: 2 different sources- A. Techniques used for the identification of fingerprints of individuals
- B. Molecular analysis of profiles of DNA samples
- C. Analysis of DNA samples using imprinting devices
- D. Both Techniques are used for the identification of fingerprints of individuals and Analysis of DNA samples using imprinting devices
Explanation: DNA fingerprinting is also called DNA typing, DNA profiling in genetics, it is the method in which a small amount of DNA material is…
Correct answer: Molecular analysis of profiles of DNA samples- A. Small size vector
- B. Large size vector
- C. Large size vector with no origin of replication.
- D. Small size vector with no origin of replication.
Explanation: In molecular cloning, a vector is a DNA molecule used as a vehicle to artificially carry foreign genetic material into another cell, where…
Correct answer: Small size vector- A. Primer
- B. Probe
- C. Restriction enzyme
- D. Taq Polymerase
Explanation: Aprobe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome.
Correct answer: Probe- A. Agarose
- B. Charge in DNA
- C. Fluorescent dye
- D. Radioactive dye
Explanation: Fluorescent dyes, also known as reactive dyes or fluorophores are used for locating dna bands.
Correct answer: Fluorescent dye- A. Ligation
- B. No ligation
- C. Tight ligation
- D. Cloning
Explanation: The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides together.
Correct answer: Ligation- A. Mutant
- B. Recombinant
- C. Crossing over
- D. All of the above
Explanation: Recombinant DNA is the hybrid DNA made by two or more DNA molecules joined together. So B is the correct option.
Correct answer: Recombinant- A. Similarities in the nucleotide uracil
- B. Similarities in the deoxyribose sugar
- C. Similarities in nucleotide base pairs
- D. RNA differences
Explanation: Similarities in the nucleotide base pairs is the correct answer. Uracil is not related to DNA and is a nucleotide of RNA.The structure of…
Correct answer: Similarities in nucleotide base pairs