Among the following, which one is commonly used as a restriction enzyme:
Correct answer: B. EcoR1
- A. pBR 101
- B. EcoR1
- C. EcoR3
- D. Both pBR 101 and EcoR3
Explanation
EcoRI is a restriction of endonuclease enzymes isolated from species. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and it is also a part of the restriction-modification system. Like all enzymes, a restriction enzyme works shape-to-shape matching. When it comes into contact with a DNA sequence with a shape that matches a part of the enzyme, called the recognition site, it wraps around the DNA and causes a break in both strands of the DNA molecule.
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