The agent which separates the two strands of DNA in PCR is?
Correct answer: C. Heat
- A. DNA ligase
- B. Primer
- C. Heat
- D. Helicase
Explanation
In PCR (polymerase chain reaction), the two strands of DNA are separated by raising the temperature. The process of heating the DNA to separate the strands is called denaturation. It allows the DNA to become single-stranded and available for further steps in PCR.
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