What will happen if a vector (plasmid) is cut with a different restriction enzyme which cuts the external DNA to be incorporated in the vector (plasmid)?
Correct answer: A. Ligation
- A. Ligation
- B. No ligation
- C. Tight ligation
- D. Cloning
Explanation
The DNA ligase catalyzes the formation of covalent phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically attached to the backbone and the complete plasmid can be transformed into bacterial cells for propagation so if vector is cut by different restrict]tion enzyme ligation occurs.
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