For the location/detection of a gene in a DNA library which of the following is used?
Correct answer: B. Probe
- A. Primer
- B. Probe
- C. Restriction enzyme
- D. Taq Polymerase
Explanation
Aprobe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a sample genome. DNA probes are important tools in diagnostics to ensure high specificity and sensitivity. They are used in molecular biology methods like PCR. Fluorescence in situ hybridization (FISH) is a laboratory technique for detecting and locating a specific DNA sequence on a chromosome. The technique relies on exposing chromosomes to a small DNA sequence called a probe that has a fluorescent molecule attached to it.
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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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