Free Biotechnology MCQs with Answers

731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.

Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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731 questions · page 22 of 37

  • A. Replication
  • B. Recombination
  • C. Gene mapping
  • D. None of these options is correct

Explanation: Option A: Replication refers to the process of copying DNA during cell division and is not directly related to gene mapping.Option B…

Correct answer: Gene mapping
  • A. RNA
  • B. Protein
  • C. Carbohydrate
  • D. DNA

Explanation: Primers are small, chemically synthesised oligonucleotides that are complementary to the sequences, present at 3' end of the template DNA.

Correct answer: RNA
  • A. Polymerase chronic reaction
  • B. Polymerase chain reaction
  • C. Polymerase copy reaction
  • D. Polymerase cross-reaction

Explanation: Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of…

Correct answer: Polymerase chain reaction
  • A. Adenosine transcriptase
  • B. Adenosine transaminase
  • C. Adenosine polymerase
  • D. Adenosine deaminase

Explanation: Adenosine deaminase deficiency (ADA deficiency) is an inherited condition that damages the immune system and is a common cause of severe…

Correct answer: Adenosine deaminase
  • A. Restriction Exonuclease
  • B. Restriction Ligase
  • C. Restriction Endonuclease
  • D. DNA polymerase

Explanation: Restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the…

Correct answer: Restriction Endonuclease
  • A. Readily incorporate cloned DNA
  • B. Capable of automatic replication
  • C. Capable of being isolated from genomic DNA
  • D. All of these

Explanation: Scientists have taken advantage of plasmids to use them as tools to clone, transfer, and manipulate genes.

Correct answer: All of these
  • A. Both sense and anti­sense RNA
  • B. A particular hormone
  • C. An antifeedant
  • D. A toxic protein

Explanation: Many nematodes live in plants and animals including human beings. a nematode Meloidogyne incognita infects tobacco plants and causes a…

Correct answer: A toxic protein
  • A. Producing a petrol­like fuel from rice
  • B. Alleviation of vitamin A deficiency
  • C. Pest resistance
  • D. Herbicide tolerance.

Explanation: Golden rice is a transgenic variety of rice (Oryza sativa) which contains good quantities of B­-carotene (provitamin A-inactive state of…

Correct answer: Alleviation of vitamin A deficiency
  • A. Malaria
  • B. Dengue
  • C. Thypoid
  • D. Tetanus

Explanation: Tetanus is a bactenal infection which can be treated by anti-tetanus serum.

Correct answer: Tetanus
  • A. Healthy Gene
  • B. Chromosome
  • C. Protein
  • D. Carbohydrate

Explanation: This option is the correct answer. In cystic fibrosis, liposomes (microscopic vesicles) can be used as a delivery system for introducing a…

Correct answer: Healthy Gene
  • A. CaCl2
  • B. NaCl
  • C. KCl
  • D. NaOH

Explanation: CaCl2: This option is the correct answer. Bacterial cells are often treated with a solution of calcium chloride (CaCl2) during a process…

Correct answer: CaCl2
  • A. Phage DNA
  • B. Genomic Library
  • C. Recombinant DNA
  • D. Gene Probe

Explanation: A gene probe is a short DNA or RNA sequence that is complementary to a specific target sequence of DNA or RNA.

Correct answer: Gene Probe
  • A. Embryonic Stem Cell mediated Transfer
  • B. Microinjection
  • C. Retro-virus mediated gene Transfer
  • D. Virus vectors

Explanation: Microinjection: This option is the correct answer. Microinjection is a technique commonly used in transgenic animal research.

Correct answer: Microinjection
  • A. Viruses
  • B. Chromosomes
  • C. Enzymes
  • D. Genes

Explanation: Enzymes are essential to DNA recombinant technology, and help modify and change DNA molecules as per need.

Correct answer: Enzymes
  • A. Restriction enzyme
  • B. Microsatellites
  • C. Macrosatellites
  • D. Probes for genetic markers

Explanation: In DNA fingerprinting, probes for genetic markers are used to identify markers such as microsatellites and thus produce distinctive…

Correct answer: Probes for genetic markers
  • A. Genetic material
  • B. Enzymes
  • C. Vectors
  • D. Probes

Explanation: Plasmids are small, circular, double-stranded DNA molecules, which are used in DNA recombinant technology by modifying the plasmid and…

Correct answer: Vectors
  • A. Polymerase chain reaction
  • B. Gene sequencing
  • C. Analyzing DNA
  • D. DNA finger printing

Explanation: It is used to replicate a few or even a singular DNA molecule into millions of copies.

Correct answer: Polymerase chain reaction
  • A. Cystic fibrosis
  • B. Hypercholesterolemia
  • C. Severe combined immunodeficiency syndrome
  • D. Parkinson's disease

Explanation: Adenosine deaminase deficiency is an inherited disorder, which causes severe combined immunodeficiency syndrome, and it causes the…

Correct answer: Severe combined immunodeficiency syndrome
  • A. Probes
  • B. Palindromes
  • C. Plasmids
  • D. Prions

Explanation: Plasmids are small, circular, double-stranded DNA molecules, which are used in DNA recombinant technology by modifying the plasmid and…

Correct answer: Plasmids
  • A. Helicase
  • B. Reverse Transcriptase
  • C. Restriction Enzyme
  • D. DNA Polymerase

Explanation: Restriction enzymes are used to separate DNA molecules at specific points, and can be used to separate specific genes.

Correct answer: Restriction Enzyme