The chemical nature of primers used in the PCR process is _.
Correct answer: A. RNA
- A. RNA
- B. Protein
- C. Carbohydrate
- D. DNA
Explanation
Primers are small, chemically synthesised oligonucleotides that are complementary to the sequences, present at 3' end of the template DNA. They hybridise to the target DNA region, one to each strand of the double helix. These primers are oriented with their ends facing each other allowing synthesis of the DNA towards one another. Thus, the primer is a short, single-stranded DNA sequence which hybridises with the template DNA and defines the region of the DNA that needs to be amplified.
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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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