Free Biotechnology MCQs with Answers

• A. Have a foreign gene inserted into them
• B. Have an important role in the large scale production of medicinal products
• C. Are considered beneficial to humans
• D. All of the above

Explanation: Transgenic organisms have all three characteristics mentioned in options A-C.

• A. dATP
• B. Primers
• C. DNA fragments
• D. Ribonucleotides

Explanation: DNA primer is not required for a PCR reaction. The reason for RNA primers to be used in PCR is the non availability of DNA primers.

• A. Na+ ions
• B. K+ ions
• C. CI- ions
• D. Ca2+ ions
• E. Mg2+ ions

Explanation: Cystic fibrosis is due to the lack of Cl- protein channels which leads to decreased movement of chloride ions outside the cell, and thus…

• A. Bacterial diseases only
• B. Viral diseases only
• C. Both Viral and Bacterial
• D. All type of disease-causing organisms

Explanation: Live, attenuated vaccines fight viruses and bacteria.Currently, there are no immunotherapeutics or vaccines approved for the treatment or…

• A. Chemically synthesized
• B. Synthesized from mRNA
• C. Synthesized by using terminating nucleotides
• D. Chemically cut into pieces

Explanation: This method is based on nucleobase-specific partial chemical modification of DNA and subsequent cleavage of the DNA backbone at sites…

• A. Sexual reproduction
• B. Asexual reproduction
• C. Both sexual and asexual
• D. None of the given options

Explanation: Cloning is an asexual method of reproduction, where fertilization or inter-gamete contact does not take place.

• A. Agar gel
• B. Agarose gel
• C. Dextran
• D. Toluene gel

Explanation: Agarose gel is used during gel electrophoresis. The restriction fragments can be separated according to their length (molecular weight or…

• A. It can be isolated from chromosome
• B. It can be synthesized chemically
• C. It can be made from protein
• D. It can be made from mRNA

Explanation: Recombinant DNA (rDNA) technology has made the transfer of genes from one organism to another possible

• A. DNA fingerprinting
• B. Polymerase Chain Reaction
• C. Gel Electrophoresis
• D. Recombinant DNA technology

Explanation: In research or diagnosis, DNA amplification can be conducted through methods such as Polymerase chain reaction; an easy, cheap, and…

• A. SCID
• B. Tuberculosis
• C. Cystic fibrosis
• D. Cancer

Explanation: Gene therapy is a medical field which focuses on the genetic modification of cells to produce a therapeutic effect or the treatment of…

• A. Insulin-producing bacteria
• B. Oil eating bacteria
• C. Photosynthetic bacteria
• D. Metal extracting bacteria

Explanation: For bacterias to be photosynthetic they do not need to be genetically programmed. There are naturally occurring photosynthetic bacterias.

• A. Group A
• B. Group B
• C. Group B and C
• D. Group C

Explanation: Group A is correct because it has all the tools required to engineer a plant cell.

• A. Cloning
• B. Recombinant DNA technique
• C. Cell culture
• D. Gel Electrophoresis

Explanation: Gel electrophoresis is a technique used to separate DNA fragments according to their size and speed.DNA samples are loaded into wells…

• A. Host cell
• B. Donor cell
• C. Restriction enzyme
• D. Recombinant DNA

Explanation: When a piece of DNA from a host is cut out to be inserted into a source DNA to form a combined DNA it is called recombinant DNA.

• A. Viruses
• B. Bacteria
• C. Eukaryotes
• D. All of these

Explanation: Restriction endonucleases are class of enzymes found in bacteria. They act on bacteriophage viruses (viruses that attack bacteria) and…

• A. Ligase
• B. Phosphatase
• C. Ribonuclease
• D. Restriction enzyme

Explanation: Restriction enzymes, also called restriction endonucleases, recognize a specific sequence of nucleotides in double stranded DNA and cut…

• A. No gene
• B. No gene in transposition
• C. Widely used in gene transfer
• D. All of these options are correct

Explanation: Genetic modification of plants involves adding a specific stretch of DNA into the plant's genome, giving it new or different…

• A. Adenosine Deaminase (ADA)
• B. Pancreatic Enzyme
• C. ß-galactosidase
• D. ß-lactamase

Explanation: 'SCID' is due to Adenosine Deaminase (ADA) deficiency so, it is administered to overcome the deficiency. Hence, option A is correct.

• A. Animals, with inferior traits
• B. Transgenic mice for testing the safety of the polio vaccine before use in humans
• C. Animals, that have the same genetic constitution
• D. None of the above

Explanation: Transgenic mice are being formed for use in testing the safety of vaccines before they are used on human beings.

• A. Streptomycin
• B. Ampicillin and Tetracycline
• C. Tetracycline and doxycycline
• D. Ampicillin

Explanation: Factual recall;pBR322 is base pairs in length and has two antibiotic resistance genes - the gene 'bla', encoding the ampicillin resistance…