Which of the following is not necessary for PCR to occur?
Correct answer: D. Ribonucleotides
- A. dATP
- B. Primers
- C. DNA fragments
- D. Ribonucleotides
Explanation
DNA primer is not required for a PCR reaction. The reason for RNA primers to be used in PCR is the non availability of DNA primers. The RNA primers complimentary to the cellular DNA are easily synthesized by the DNA Primase enzyme which is nothing but RNA polymerase just like mRNA.
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