The introduction of t-­DNA into plants involves:

Explanation

Ti plasmid (tumor inducing) from the soil bacterium Agrobacterium tumefaciens is effectively used as a vector for gene transfer to plant cells. The part of Ti plasmid transferred into plant cell DNA is called the T­-DNA. This T-­DNA, with desired DNA spliced into it, is inserted into the chromosomes of the host plant where it produces copies of itself, by migrating from one chromosomal position to another at random. Such plant cells are then cultured, induced to multiply, and then differentiate to form plantlets. Transferred into the soil, the plantlets grow into mature plants, carrying the foreign gene, expressing throughout the new plant.