Free Biotechnology MCQs with Answers

• A. 'Flavr Savr' tomato
• B. Canolla
• C. Golden rice
• D. Bt­-Brinjal

Explanation: Golden rice is a transgenic variety of rice (Oryza sativa) that contains good quantities of B-carotene (provitamin A-inactive state of…

• A. The relative proportions of purines and pyrimidines in DNA
• B. The relative difference in the DNA occurrence in blood, skin, and saliva
• C. The relative amount of DNA in the ridges and grooves of the fingerprints
• D. Satellite DNA occurring as highly repeated, short DNA segments

Explanation: DNA fingerprinting is a technique of determining nucleotide sequences of certain areas of DNA which are unique to each individual.

• A. Diabetes mellitus
• B. Chicken pox
• C. Rheumatoid arthritis
• D. Adenosine deaminase deficiency.

Explanation: Gene therapy is a collection of methods that allows the correction of a gene defect that has been diagnosed in a child/embryo.

• A. Nematodes
• B. Fungi
• C. Viruses
• D. Insects

Explanation: Many nematodes live in plants and animals including human beings. A nematode Meloidogyne incognita infests the roots of tobacco plants and…

• A. Binds with epithelial cells in the midgut of the insect pest ultimately killing it
• B. Is coded by several genes including the gene cry
• C. Is activated by acid pH of the foregut of the insect pest
• D. Does not kill the carrier bacterium which is itself resistant to this toxin

Explanation: Soil bacterium Bacillus thuringiensis produces proteins that kill certain insects like lepidopterans (tobacco budworm, armyworm)…

• A. B and C
• B. C and D
• C. A and C
• D. A and B

Explanation: In 1997, the first transgenic cow, Rosie, produced human protein enriched milk.

• A. Long fibre and resistance to aphids
• B. Medium yield, long fibre and resistance to beetle pests
• C. High yield and production of toxic protein crystals which kill dipteran pests
• D. High yield and resistance to bollworms.

Explanation: Bt toxin genes were isolated from Bacillus thuringiensis and incorporated into the cotton plant.

• A. Nicotine
• B. Morphine
• C. Quinine
• D. Insulin

Explanation: Insulin is now being commercially produced by genetic engineering. Insulin consists of two short polypeptide chains: chain A and chain B…

• A. Bt protein exists as active toxin in the Bacillus
• B. The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication
• C. The concerned Bacillus has antitoxins
• D. The inactive protoxin gets converted into active form in the insect gut

Explanation: Soil bacterium Bacillus thuringiensis produces proteins that kill certain insects like lepidopterans (tobacco budworm, armyworm)…

• A. Generated by introducing foreign DNA into a cell and regenerating a plant from that cell
• B. Produced after protoplast fusion in artificial medium
• C. Grown in artificial medium after hybridization in the field
• D. Produced by a somatic embryo in artificial medium

Explanation: The plants produced through genetic engineering contain genes, usually from an unrelated organism.

• A. Insecticide
• B. Agent for production of dairy products
• C. Source of industrial enzyme
• D. Indicator of water pollution

Explanation: Soil bacterium Bacillus thuringiensis produces proteins that kill certain insects like lepidopterans (tobacco budworm, armyworm)…

• A. Encourage eco­friendly herbicides
• B. Reduce herbicide accumulation in food articles for health safety
• C. Eliminate weeds from the field without the use of manual labour
• D. Eliminate weeds from the field without the use of herbicides.

Explanation: Genetic engineering has helped to develop such transgenic crop plants which are resistant to herbicides so that they are not damaged when…

• A. Rhizobium
• B. Saccharomyces
• C. Escherichia
• D. Mycobacterium

Explanation: Insulin is now being commercially produced by genetic engineering. Insulin consists of two short polypeptide chains: chain A and chain B…

• A. Trichoderma
• B. Xanthomonas
• C. Bacillus
• D. Pseudomonas.

Explanation: Bioremediation is the process of using living micro­organisms to clean up a contaminated site.

• A. Biofertilizers
• B. Bio­-metallurgical techniques
• C. Bio­-mineralization processes
• D. Bioinsecticidal plants.

Explanation: Bacillus thuringiensis strains have been used for designing bioinsecticidal plants.

• A. The human chromosome can replicate in bacterial cell
• B. The mechanism of gene regulation is identical in humans and bacteria
• C. Bacterial cell can carry out the RNA splicing reactions
• D. The genetic code is universal.

Explanation: Genetic code may be defined as the sequence of nucleotides in polynucleotide chain which determines the sequence of amino acids in a…

• A. Insect resistance
• B. High lysine (essential amino acid) content
• C. High protein content
• D. High vitamin­-A content

Explanation: Golden rice is a transgenic crop rice with high vitamin A content. It has been developed by transferring beta carotene synthesizing gene…

• A. Molecular analysis of profiles of DNA samples
• B. Analysis of DNA samples using imprinting devices
• C. Techniques used for molecular analysis of different specimens of DNA
• D. Techniques used for identification of fingerprints of individuals

Explanation: DNA fingerprinting is a technique of determining nucleotide sequences of certain areas of DNA which are unique to each individual.

• A. Azotobacter
• B. Rhizobium of the roots of leguminous plants
• C. Agrobacterium
• D. Yeast as a 2 mm plasmid

Explanation: Ti plasmid (tumor inducing) from the soil bacterium Agrobacterium tumefaciens is effectively used as a vector for gene transfer to plant…

• A. Alkaline phosphatase
• B. Catalase
• C. DNA probe
• D. RNAase.

Explanation: ELISA (enzyme linked immunosorbent assay) screening test is the initial test to diagnose AIDS.