The Ti plasmid is often used for making transgenic plants. The plasmid is found in:
Correct answer: C. Agrobacterium
- A. Azotobacter
- B. Rhizobium of the roots of leguminous plants
- C. Agrobacterium
- D. Yeast as a 2 mm plasmid
Explanation
Ti plasmid (tumor inducing) from the soil bacterium Agrobacterium tumefaciens is effectively used as a vector for gene transfer to plant cells. The part of Ti plasmid transferred into plant cell DNA is called the T-DNA. This T-DNA with desired DNA spliced into it is inserted into the chromosomes of the host plant where it produces copies of itself, by migrating from one chromosomal position to another at random. Such plant cells are then cultured, induced to multiply and differentiate to form plantlets. Transferred into the soil, the plantlets grow into mature plants, carrying the foreign gene, expressed throughout the new plant.
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