Free Biotechnology and Health Care MCQs with Answers

233 Biotechnology and Health Care MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.

Biotechnology applies genetic engineering, cell culture and recombinant DNA methods to medicine and public health. The material includes production of insulin, vaccines and antibiotics, diagnostic techniques, gene therapy, genetically modified organisms and the ethical concerns surrounding manipulation of human genes.

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233 questions · page 11 of 12

  • A. Exonucleases
  • B. Endonucleases
  • C. Polymerases
  • D. Reverse transcriptases

Explanation: Endonucleases also known as restriction enzymes are molecular scissors as they cleave DNA at or near specific sequences known as…

Correct answer: Endonucleases
  • A. Heating→ Cooling → Add Primer→ Copying of strand
  • B. Heating→ Add Primer→ Cooling→ Copying of strand
  • C. Add Primer →Heating→ Cooling →Copying of strand
  • D. Cooling→ Add Primer→ Heating →Copying of strand

Explanation: Polymerase chain Reaction The polymerase chain reaction is a method widely used to rapidly make millions to billions of copies of a…

Correct answer: Heating→ Cooling → Add Primer→ Copying of strand
  • A. Complementary DNA
  • B. Mutated DNA
  • C. Recombinant DNA
  • D. Cloned DNA

Explanation: A recombinant Dna is a dna molecule from two different species that are inserted into host organism to produce new genetic combinations…

Correct answer: Recombinant DNA
  • A. lone DNA
  • B. Recombinant DNA
  • C. cDNA
  • D. rDNA

Explanation: Recombination is the process through which a new gene is inserted into a bacterial DNA "The plasmid".

Correct answer: Recombinant DNA
  • A. Nitrogenous base
  • B. Base sugar
  • C. Phosphodiester bond
  • D. Hydrogen bond

Explanation: The restriction endonucleases cuts the two strands of the double helix at specific point in the sugar phosphate backbone of DNA, it breaks…

Correct answer: Phosphodiester bond
  • A. Endonuclease
  • B. Urease
  • C. Ligase
  • D. Helicase

Explanation: Ligase is also known as molecular glue, responsible for the binding of two molecules, using ATP.

Correct answer: Ligase
  • A. Blunt ends
  • B. Non-palindromic ends
  • C. Sticky ends
  • D. Split ends

Explanation: EcoRI is a restriction enzyme. Restriction enzymes either make staggered cuts or blunt cuts.

Correct answer: Sticky ends
  • A. Fungi
  • B. Algae
  • C. Bacteria
  • D. Viruses

Explanation: Restriction endonucleases are also called restriction enzymes. They are naturally found in bacteria where they serve a host-defense role.

Correct answer: Bacteria
  • A. Western blotting
  • B. Northern blotting
  • C. Autoradiography
  • D. Gel electrophoresis

Explanation: Gel electrophoresis is a technique used in molecular biology to separate different-sized fragments of charge-bearing polymers (proteins…

Correct answer: Gel electrophoresis
  • A. Taq-isomerase
  • B. Taq-helicase
  • C. Taq-polymerase
  • D. Taq SSBp

Explanation: Taq-polymerase is a polymerase enzyme extracted from the heat-stable bacteria called Thermus aquaticus which lives in hot springs.

Correct answer: Taq-polymerase
  • A. Trans-membrane carrier
  • B. Cl- ions
  • C. Na+ ions
  • D. Mucus membrane

Explanation: The trans-membrane carrier is responsible for allowing Chlorine ions to enter into the mucus so that water may follow due to osmosis…

Correct answer: Trans-membrane carrier
  • A. Probes
  • B. Restriction endonuclease
  • C. Mutated DNA
  • D. Recombinant DNA

Explanation: Recombinant DNA (or rDNA) is made by combining DNA from two or more sources.DNA probes are stretches of single-stranded DNA used to detect…

Correct answer: Recombinant DNA
  • A. Quina quina
  • B. Lathyrus plant
  • C. Calotropis plant
  • D. Cinchona plant

Explanation: Fact. Quinine is an alkaloid derived from the bark of the South American cinchona tree.

Correct answer: Cinchona plant
  • A. 100°C
  • B. 100°C for 30 min
  • C. 71°C for 15 min and 62°C for 32 min
  • D. 71°C for 32 min and 62°C for 15 sec

Explanation: Pasteurization is a food processing method where a mild heat treatment is applied to food to kill harmful bacteria (pathogens) and extend…

Correct answer: 71°C for 15 min and 62°C for 32 min
  • A. Parents who gestated and gave birth to Dolly
  • B. Parents, who donated egg cell
  • C. Parent, who donated somatic cell
  • D. Both b and c

Explanation: The correct answer is Option D: Both b and c. Dolly, the cloned sheep, was identical to the sheep whose somatic cell was used for cloning…

Correct answer: Both b and c
  • A. 8
  • B. 12
  • C. 16
  • D. 20

Explanation: Primers in PCR are typically designed to be 18-22 bases long. This length allows them to bind specifically to the target DNA sequence…

Correct answer: 20
  • A. Gave birth to the dolly
  • B. Donated reproductive cells
  • C. Donated somatic cell
  • D. Both A and B

Explanation: Dolly, the cloned baby sheep, was created using a somatic cell nuclear transfer (SCNT) technique.

Correct answer: Donated somatic cell
  • A. Treating anemia
  • B. Bone marrow transplant
  • C. Dissolving blood clot
  • D. Treatment of cancer

Explanation: Tissue plasminogen activator (TPA) is primarily used for dissolving blood clots in medical emergencies.

Correct answer: Dissolving blood clot
  • A. Four Parents
  • B. Three Parents
  • C. Two parents
  • D. One Parent only

Explanation: Cloning is a mode of asexual reproduction through which genetically identical copies of an organism are produced.

Correct answer: One Parent only
  • A. Genetic Engineering
  • B. Hybridization
  • C. Inbreeding
  • D. Sequencing

Explanation: Hybridization involves the crossing of dissimilar individuals to bring together the best of both parents in the offspring.

Correct answer: Hybridization