Which of the following is the correct sequence of PCR?
Correct answer: A. Heating→ Cooling → Add Primer→ Copying of strand
- A. Heating→ Cooling → Add Primer→ Copying of strand
- B. Heating→ Add Primer→ Cooling→ Copying of strand
- C. Add Primer →Heating→ Cooling →Copying of strand
- D. Cooling→ Add Primer→ Heating →Copying of strand
Explanation
Polymerase chain Reaction The polymerase chain reaction is a method widely used to rapidly make millions to billions of copies of a specific DNA sample in a short period. Process PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified, and then multiple rounds of DNA synthesis to amplify that segment. The process has 4 steps in total. Sequence The correct sequence of PCR is Heating it breaks the double-stranded structure Cooling Adding the primer which then annealed to make a copy of the strand. Opt A is the right choice here.
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