Asked in UHS MDCAT 2017 2017Moderate

Restriction enzyme EcoR1 cuts DNA to produce:

Correct answer: C. Sticky ends

  • A. Blunt ends
  • B. Non-palindromic ends
  • C. Sticky ends
  • D. Split ends

Explanation

EcoRI is a restriction enzyme. Restriction enzymes either make staggered cuts or blunt cuts. A staggered cut is one in which the duplex fragment shows single-stranded projected ends called sticky ends. Blunt cuts do not produce sticky ends.

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