The primers used in polymerase chain reaction has a sequence of bases
Correct answer: D. 20
- A. 8
- B. 12
- C. 16
- D. 20
Explanation
Primers in PCR are typically designed to be 18-22 bases long. This length allows them to bind specifically to the target DNA sequence, providing both high specificity and efficiency at the annealing temperature. Options A, B, and C (8, 12, and 16 bases, respectively) are generally too short to offer the necessary specificity and might lead to non-specific binding or inefficient amplification. Therefore, option D, 20 bases, is the correct choice as it falls within the optimal range for PCR primer design.
This question appeared on the ETEA MDCAT 2009 paper, which you can sit online with every answer explained.
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