Free Biotechnology MCQs with Answers

731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.

Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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731 questions · page 9 of 37

  • A. X-ray
  • B. Ultrasound
  • C. Amniocentesis
  • D. Complete Blood Count

Explanation: The correct answer is amniocentesis. This procedure involves extracting a small amount of amniotic fluid, which contains fetal cells, to…

Correct answer: Amniocentesis
  • A. Okazaki fragment
  • B. Palindromic sequences
  • C. Promoter sequences
  • D. Primer

Explanation: In molecular biology, palindromic sequences are referred to as the sequence of nucleotides in the DNA duplex or RNA, where the sequence in…

Correct answer: Palindromic sequences
  • A. RNA polymerase
  • B. DNA polymerase
  • C. Taq polymerase
  • D. DNA ligase

Explanation: Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as…

Correct answer: Taq polymerase
  • A. Animal husbandry
  • B. Wild life management
  • C. Hybrid breeding
  • D. Livestock management

Explanation: The study of the proper care, breeding, and management of economically important domesticated animals is known as animal husbandry.

Correct answer: Animal husbandry
  • A. Antiseptics
  • B. Disinfectants
  • C. Pasteurization
  • D. Antibiotics

Explanation: Pasteurization is indeed a physical method used to control bacteria in food and beverages.

Correct answer: Pasteurization
  • A. Restriction endonuclease-production of RFLP
  • B. DNA ligase-production of sticky ends in restriction fragments
  • C. Reverse transcription-production of cDNA
  • D. PCR-gene amplification

Explanation: PCR is a very sensitive technique that allows rapid amplification of a specific segment of DNA.

Correct answer: PCR-gene amplification
  • A. Gel electrophoresis
  • B. RFLP analysis
  • C. Polymerase chain reaction
  • D. Tissue culture

Explanation: PCR is a technique used to amplify a specific region of DNA, generating millions of copies of that region.

Correct answer: Polymerase chain reaction
  • A. Produce cDNA
  • B. Cut plasmid DNA
  • C. Join DNA at sticky ends
  • D. Clone DNA

Explanation: In genetic engineering, restriction enzymes are used for cutting DNA fragments.

Correct answer: Cut plasmid DNA
  • A. Each cell has full genetic potential of the organism
  • B. A single cell could become a complete plant
  • C. Because they have lesser potential for tissue culturing
  • D. Both A and B

Explanation: Each cell has the full genetic potential of the organism, and a single cell could become a complete plant.

Correct answer: Both A and B
  • A. Thermus aquaticus
  • B. Thermus floral
  • C. Floral aquaticus
  • D. Taq aquaticus

Explanation: Taq polymerase is a heat-stable DNA polymerase that is used in the polymerase chain reaction (PCR) to amplify DNA.

Correct answer: Thermus aquaticus
  • A. Electrophoresis
  • B. Micrometry
  • C. Microscopy
  • D. Chromatography
  • E. Spectrophotometry

Explanation: Spectrophotometry is a quantitative analytical technique used to measure the absorbance or transmittance of light by a substance.

Correct answer: Spectrophotometry
  • A. Gene therapy
  • B. Cloning
  • C. Tissue culture
  • D. Gene sequencing

Explanation: Gene therapy is a technique that uses a gene(s) to treat, prevent or cure a disease or medical disorder.

Correct answer: Gene therapy
  • A. Hypoxanthine-guanine transferase
  • B. Xanthine oxidase
  • C. PRPP synthetase
  • D. Adenosine deaminase

Explanation: ADA deficiency leads to SCID, a group of rare, inherited disorders characterized by a severe impairment of both the T and B lymphocytes of…

Correct answer: Adenosine deaminase
  • A. Animals have been used
  • B. Bacteria have been used
  • C. Plants have been used
  • D. Viruses have been used

Explanation: Genetically engineerad bacteria are used to remove environmental pollutants through bioremediation.

Correct answer: Bacteria have been used
  • A. Biotechnology
  • B. Bioenergetics
  • C. Genetic engineering
  • D. Gene therapy

Explanation: The use of natural biological system to produce a product or to achieve rend desired by humans is called biotechnology.

Correct answer: Biotechnology
  • A. Oral cavity
  • B. Nostrils
  • C. Thoracic cavity
  • D. Mouth

Explanation: For treatment of cystic fibrosis, a solution containing gene coated liposomes is sprayed into patient's nostrils.

Correct answer: Nostrils
  • A. Gene therapy
  • B. Transplant therapy
  • C. Chemotherapy
  • D. Radiation therapy

Explanation: In Parkinson's disease, dopamine-producing cells can be directly transplanted into brain to restore dopamine levels, which is known as…

Correct answer: Transplant therapy
  • A. Liposomes
  • B. Lysosomes
  • C. Nucleosomes
  • D. Peroxisomes

Explanation: Liposomes are spherical vesicles of of phospholipid bilayer which are used as a vehicle for administration of nutrients and pharmaceutical…

Correct answer: Liposomes
  • A. SCIDS
  • B. Cystic fibrosis
  • C. Hypercholesterolemia
  • D. Cardiovascular disorder

Explanation: In this gene therapy, liver cells are infected with a retrovirus carrying the normal LDL receptor gene.

Correct answer: Hypercholesterolemia
  • A. Bacteriophage
  • B. Retrovirus
  • C. Lambda virus
  • D. Bacterium

Explanation: The retrovirus acts as a vector for gene therapy.

Correct answer: Retrovirus