Which one of the following recombinant DNA technology tool is incorrectly paired with its use?
Correct answer: D. PCR-gene amplification
- A. Restriction endonuclease-production of RFLP
- B. DNA ligase-production of sticky ends in restriction fragments
- C. Reverse transcription-production of cDNA
- D. PCR-gene amplification
Explanation
PCR is a very sensitive technique that allows rapid amplification of a specific segment of DNA. PCR makes billions of copies of a specific DNA fragment or gene, which allows detection and identification of gene sequences using visual techniques based on size and charge.
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Biotechnology applies genetic engineering, cell culture and recombinant DNA methods to medicine and public health. The material includes production of insulin, vaccines and antibiotics, diagnostic techniques, gene therapy, genetically modified organisms and the ethical concerns surrounding manipulation of human genes.
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