Free Biotechnology MCQs with Answers
731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.
Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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- A. Fatal heart attack at old age
- B. A curable heart attack at old age
- C. A curable heart attack at young age
- D. Fatal heart attack at young age
Explanation: High levels of cholesterol especially LDL can lead to early atherosclerosis, which increases the risk of a fatal heart attack at a young…
Correct answer: Fatal heart attack at young age- A. Ex - vivo and in vivo
- B. Ex vivo - Ex vitro
- C. Ex - vivo and in vitro
- D. In vitro - Ex vitro
Explanation: In ex-vivo method cells or tissurs are taken out of the body and after insersion of normal genes they are implanted in the body.
Correct answer: Ex - vivo and in vivo- A. Cancer
- B. Cardiovascular diseases
- C. Cancer and cardiovascular diseases
- D. Tetanus
Explanation: Gene therapy involves introducing healthy genes into a patient's cells to replace or repair faulty genes, helping treat serious illnesses…
Correct answer: Cancer and cardiovascular diseases- A. Only females produce milk
- B. Females don't produce milk until maturity
- C. Each animal urinates throughout life
- D. It is easier to extract proteins from urine than from milk
Explanation: Urine is preferred because both sexes produce it throughout life, unlike milk, but extracting proteins from urine is actually.
Correct answer: It is easier to extract proteins from urine than from milk- A. Prothrombin
- B. Antithrombin-III
- C. Heparin
- D. Fibrin
Explanation: It is an anti-clotting factor used in microsurgeries to discourage formation of clot at site of surgery.
Correct answer: Antithrombin-III- A. Cows
- B. Goats
- C. Mice
- D. Squirrels
Explanation: The scientists of United States Department of Agriculture have been able to genetically engineer mice to produce human growth hormone in…
Correct answer: Mice- A. Transgenic animal
- B. Transgender animal
- C. Clone
- D. Transsexual animal
Explanation: A transgenic animal is the one which has a foreign gene inserted in it.
Correct answer: Transgenic animal- A. No escape to the wild
- B. Mutualistic help to the wild
- C. Easy escape to the wild
- D. Symbiotic help to the wild
Explanation: Due to their massive size and more vigour the wild flora fall easy prey to them.
Correct answer: No escape to the wild- A. It can work at high speed
- B. It can withstand high denaturation temperature
- C. It can withstand low denaturation temperature
- D. It can be used again and again
Explanation: DNA polymerase from Thermus aquaticus (Taq polymerase) is thermostable, so it does not denature at the high temperatures (-95 °C) used…
Correct answer: It can withstand high denaturation temperature- A. Add more enzyme
- B. Interrupt the process to add more enzyme
- C. Interrupt the process
- D. Use high temperature
Explanation: A high temperature is maintained to discourage duplex formation and chain reaction proceeds in which same enzyme is used again and again.
Correct answer: Interrupt the process to add more enzyme- A. One part in a million
- B. Less than one part in a million
- C. More than one part in a million
- D. Two parts in a million
Explanation: PCR (Polymerase Chain Reaction) is highly specific and can amplify a targeted DNA sequence even if it is present at less than one part in…
Correct answer: Less than one part in a million192. In PCR, primers are sequences of 20 bases that are complementary to the bases on either side of the:
- A. Target DNA
- B. DNA polymerase
- C. Primase
- D. RNA polymerase
Explanation: In PCR, primers bind to sequences flanking the target DNA to initiate replication.
Correct answer: Target DNA- A. Heating DNA for one minute to denature
- B. Cooling for two minutes and adding primer
- C. Addition of DNA polymerase and waiting for 1.5 minutes
- D. Recycling
Explanation: The second step of PCR is annealing, where the reaction is cooled so primers can bind to the single-stranded DNA.
Correct answer: Cooling for two minutes and adding primer- A. Bioreactor
- B. Test tube
- C. Expression system
- D. Petridish
Explanation: PCR amplifies DNA in vitro, meaning it happens in a test tube, not inside cells or dishes.
Correct answer: Test tube- A. DNA polymerase
- B. DNA helicase
- C. DNA ligase
- D. DNA primase
Explanation: DNA ligase is the anzyme that joins DNA fragments by forming phosphodiester bonds, effectively sealing a foreign piece of DNA into a vector.
Correct answer: DNA ligase- A. DNA of bacteria
- B. DNA of plant viruses
- C. DNA of bacterial viruses (bacteriophages)
- D. DNA of animal viruses
Explanation: Bacteriophages, or bacterial viruses, are efficient vectors for introducing genetic material into bacteria due to their natural ability to…
Correct answer: DNA of bacterial viruses (bacteriophages)- A. Restriction enzymes
- B. Plasmids
- C. DNA ligases
- D. Sticky ends
Explanation: Plasmids were discovered by investigators studying the sex life of the intestinal bacterium Escherichia coli.
Correct answer: Plasmids198. For preparation of recombinant DNA, the plasmid is cut with the same enzyme which was used for:
- A. Isolation of the gene of interest
- B. Cutting all such plasmids
- C. Cutting any piece of DNA
- D. Cutting extrachromosomal DNA
Explanation: The same restriction enzyme is used to cut both the plasmid and the gene of interest to create compatible onds for ligation.
Correct answer: Isolation of the gene of interest- A. Reverse transcriptase
- B. Helicases
- C. Ligase
- D. Restriction endonucleases
Explanation: A restriction enzyme, restriction endonuclease, REase, ENase or restrictase enzyme that cleaves DNA into fragments at or near specific…
Correct answer: Restriction endonucleases- A. Faecal
- B. Intestinal
- C. Milk
- D. Soil
Explanation: Escherichia coli is predominantly found in the intestines of humans animals, which is why it is referred to as an intestinal bacterium.
Correct answer: Intestinal