For preparation of recombinant DNA, the plasmid is cut with the same enzyme which was used for:
Correct answer: A. Isolation of the gene of interest
- A. Isolation of the gene of interest
- B. Cutting all such plasmids
- C. Cutting any piece of DNA
- D. Cutting extrachromosomal DNA
Explanation
The same restriction enzyme is used to cut both the plasmid and the gene of interest to create compatible onds for ligation.
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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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