Moderate

The second step of PCR technique is:

Correct answer: B. Cooling for two minutes and adding primer

  • A. Heating DNA for one minute to denature
  • B. Cooling for two minutes and adding primer
  • C. Addition of DNA polymerase and waiting for 1.5 minutes
  • D. Recycling

Explanation

The second step of PCR is annealing, where the reaction is cooled so primers can bind to the single-stranded DNA.

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