Free Biotechnology MCQs with Answers
731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.
Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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Read the Biotechnology notesFree MDCAT chapter notes with key terms731 questions · page 36 of 37
701. When two different pieces of DNA are joined together, the result is which one of the following?
- A. Complementary DNA
- B. Mutated DNA
- C. Recombinant DNA
- D. Cloned DNA
Explanation: A recombinant Dna is a dna molecule from two different species that are inserted into host organism to produce new genetic combinations…
Correct answer: Recombinant DNA- A. lone DNA
- B. Recombinant DNA
- C. cDNA
- D. rDNA
Explanation: Recombination is the process through which a new gene is inserted into a bacterial DNA "The plasmid".
Correct answer: Recombinant DNA- A. Nitrogenous base
- B. Base sugar
- C. Phosphodiester bond
- D. Hydrogen bond
Explanation: The restriction endonucleases cuts the two strands of the double helix at specific point in the sugar phosphate backbone of DNA, it breaks…
Correct answer: Phosphodiester bond- A. Endonuclease
- B. Urease
- C. Ligase
- D. Helicase
Explanation: Ligase is also known as molecular glue, responsible for the binding of two molecules, using ATP.
Correct answer: Ligase- A. Blunt ends
- B. Non-palindromic ends
- C. Sticky ends
- D. Split ends
Explanation: EcoRI is a restriction enzyme. Restriction enzymes either make staggered cuts or blunt cuts.
Correct answer: Sticky ends- A. Fungi
- B. Algae
- C. Bacteria
- D. Viruses
Explanation: Restriction endonucleases are also called restriction enzymes. They are naturally found in bacteria where they serve a host-defense role.
Correct answer: Bacteria- A. Western blotting
- B. Northern blotting
- C. Autoradiography
- D. Gel electrophoresis
Explanation: Gel electrophoresis is a technique used in molecular biology to separate different-sized fragments of charge-bearing polymers (proteins…
Correct answer: Gel electrophoresis- A. Taq-isomerase
- B. Taq-helicase
- C. Taq-polymerase
- D. Taq SSBp
Explanation: Taq-polymerase is a polymerase enzyme extracted from the heat-stable bacteria called Thermus aquaticus which lives in hot springs.
Correct answer: Taq-polymerase- A. Trans-membrane carrier
- B. Cl- ions
- C. Na+ ions
- D. Mucus membrane
Explanation: The trans-membrane carrier is responsible for allowing Chlorine ions to enter into the mucus so that water may follow due to osmosis…
Correct answer: Trans-membrane carrier- A. Probes
- B. Restriction endonuclease
- C. Mutated DNA
- D. Recombinant DNA
Explanation: Recombinant DNA (or rDNA) is made by combining DNA from two or more sources.DNA probes are stretches of single-stranded DNA used to detect…
Correct answer: Recombinant DNA- A. Quina quina
- B. Lathyrus plant
- C. Calotropis plant
- D. Cinchona plant
Explanation: Fact. Quinine is an alkaloid derived from the bark of the South American cinchona tree.
Correct answer: Cinchona plant- A. 100°C
- B. 100°C for 30 min
- C. 71°C for 15 min and 62°C for 32 min
- D. 71°C for 32 min and 62°C for 15 sec
Explanation: Pasteurization is a food processing method where a mild heat treatment is applied to food to kill harmful bacteria (pathogens) and extend…
Correct answer: 71°C for 15 min and 62°C for 32 min- A. Parents who gestated and gave birth to Dolly
- B. Parents, who donated egg cell
- C. Parent, who donated somatic cell
- D. Both b and c
Explanation: The correct answer is Option D: Both b and c. Dolly, the cloned sheep, was identical to the sheep whose somatic cell was used for cloning…
Correct answer: Both b and c- A. 8
- B. 12
- C. 16
- D. 20
Explanation: Primers in PCR are typically designed to be 18-22 bases long. This length allows them to bind specifically to the target DNA sequence…
Correct answer: 20- A. Gave birth to the dolly
- B. Donated reproductive cells
- C. Donated somatic cell
- D. Both A and B
Explanation: Dolly, the cloned baby sheep, was created using a somatic cell nuclear transfer (SCNT) technique.
Correct answer: Donated somatic cell- A. Treating anemia
- B. Bone marrow transplant
- C. Dissolving blood clot
- D. Treatment of cancer
Explanation: Tissue plasminogen activator (TPA) is primarily used for dissolving blood clots in medical emergencies.
Correct answer: Dissolving blood clot- A. Four Parents
- B. Three Parents
- C. Two parents
- D. One Parent only
Explanation: Cloning is a mode of asexual reproduction through which genetically identical copies of an organism are produced.
Correct answer: One Parent only- A. Genetic Engineering
- B. Hybridization
- C. Inbreeding
- D. Sequencing
Explanation: Hybridization involves the crossing of dissimilar individuals to bring together the best of both parents in the offspring.
Correct answer: Hybridization- A. mutagenic
- B. transgenic
- C. glones
- D. sequencing
Explanation: An organism whose genome has been altered by the introduction of one or more foreign DNA molecules from another species by artificial…
Correct answer: transgenic- A. They cut DNA at specific base sequences
- B. They cut DNA at multiple random sites
- C. They help in joining the pieces of DNA
- D. They are a type of nuclease
Explanation: Restriction enzymes, also known as restriction endonucleases, are proteins that cut DNA at specific sequences called restriction sites.
Correct answer: They cut DNA at specific base sequences