Free Biotechnology MCQs with Answers
731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.
Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
Last updated
Read the Biotechnology notesFree MDCAT chapter notes with key terms731 questions · page 32 of 37
- A. Heating DNA for one minute to denature
- B. Cooling for two minutes and adding primer
- C. Addition of DNA polymerase and waiting for 1.5 minutes
- D. Recycling
Explanation: In third step of PCR technique, DNA polymerase is added and then we wait for 1.5 minutes.
Correct answer: Addition of DNA polymerase and waiting for 1.5 minutes622. PCR can create:
- A. Thousands of copies of a single gene
- B. Millions of copies of a single gene
- C. Hundreds of copies of a single gene
- D. Tens of copies of a single gene
Explanation: As it is less time taking and more efficient method in which a chain reaction is generated.
Correct answer: Millions of copies of a single gene- A. Thousand of copies of desired DNA
- B. Millions of copies of desired DNA
- C. Hundreds of copies of desired DNA
- D. Billions of copies of desired DNA
Explanation: PCR can create millions of copies of a single gene or any specific piece of DNA quickly in test tube.
Correct answer: Millions of copies of desired DNA- A. Gene product must be available
- B. Vector must be available
- C. Primers must be available
- D. Bacteriophage must be available
Explanation: As DNA polymerase cannot initiate a polynucleotide chain, rather it can elongate a polynucleotide chain.
Correct answer: Primers must be available- A. Continues
- B. Initiates
- C. Extends
- D. Continues and extends
Explanation: It starts a chain reaction and keep on carrying out replication until test tube is filled.
Correct answer: Continues and extends- A. Primers bind by complementary base pairing
- B. The target DNA duplex is unwound
- C. The target DNA gets denatured
- D. The primers get separated from target DNA
Explanation: In PCR both strands of DNA act as template for new strands to be synthesized.
Correct answer: Primers bind by complementary base pairing- A. Hot springs
- B. Hot pools
- C. Hot thermal vents
- D. Hot ponds
Explanation: As these are thermal bacteria,they are adapted to survive in high temperature.
Correct answer: Hot springs- A. Manual microinjection
- B. Vortex mixing
- C. Manual microinjection or vortex mixing
- D. Using electric current
Explanation: These are two available methods to insert a desired gene into an animal egg.
Correct answer: Manual microinjection or vortex mixing- A. Fishes and cows
- B. Rabbits and sheeps
- C. Cows and pigs
- D. Fishes, cows, pig, rabbits and sheep
Explanation: It is possible by tempering their genetic makeup.
Correct answer: Fishes, cows, pig, rabbits and sheep- A. Therapeutic
- B. Therapeutic and diagnostic
- C. Diagnostic
- D. Osmotic
Explanation: Some proteins are used for treatment of diseases. They are called therapeutic proteins and diagnostic proteins as they are used to…
Correct answer: Therapeutic and diagnostic- A. Cystic fibrosis
- B. Blood diseases
- C. Cancer
- D. Cystic fibrosis, Cancer & Blood diseases
Explanation: In future it is being envisioned to get drugs prepared by transgenic animals against cystic fibrosis, cancer and blood diseases.
Correct answer: Cystic fibrosis, Cancer & Blood diseases- A. Amplified in mature specialized cells
- B. Turned off in mature specialized cells
- C. Lost in mature specialized cells
- D. Mutated in mature specialized cells
Explanation: When a cell is assigned a specialized job, only those genes remain active which are associated with that particular job, rest are turned…
Correct answer: Turned off in mature specialized cells- A. Genetic engineering
- B. Gene therapy
- C. Biotechnology
- D. Gene mutation
Explanation: Treating some genetic disorder by replacing a faulty gene with normal gene is called gene therapy.
Correct answer: Gene therapy- A. Adenosine deaminase
- B. Homogentisic and hydroxylase
- C. Phenylalanine hydroxylase
- D. Homogentisic and dehydrogenase
Explanation: Such children lack an enzyme adenosine deaminase (ADA) that is involved in the maturation of T and B cells and therefore, they are…
Correct answer: Adenosine deaminase- A. Phenyl alanine hydroxylase
- B. ADA
- C. Homogentisic acid dehydrogenase
- D. Phospho hexokinase
Explanation: ADA (adenosine deaminase) is involved in the maturation of T and B cells and therefore, the person lacking it are subjected to life…
Correct answer: ADA636. For treatment of SCIDS, the bone marrow stem cells are infected with a retrovirus that carries:
- A. A normal enzyme
- B. A mutant gene for the enzyme
- C. A normal gene for the enzyme
- D. A normal gene for T and B cells
Explanation: Retrovirus acts a vector and carrier a normal gene for the synthesis of ADA enzyme with its DNA (recombinant DNA).
Correct answer: A normal gene for the enzyme- A. They are larger in size
- B. They are numerous in number
- C. They divide to produce more cells with same gene
- D. They store much enzyme inside them
Explanation: Stem cells keep on dividing by mitosis and in this way the number is continuously added up which gradually overcome the immune deficiency.
Correct answer: They divide to produce more cells with same gene- A. SCIDS
- B. Cystic fibrosis
- C. Hypercholesterolemia
- D. Parkinsonism
Explanation: Cystic fibrosis patients lack a gene that codes for trans-membrane carrier of chloride ions.
Correct answer: Cystic fibrosis- A. SCIDS
- B. Cystic fibrosis
- C. Hypercholesterolemia
- D. Familial hypercholesterolemia
Explanation: The gene is introduced into the nasal sinus by coating it on liposomes which are sprayed just like inhalers in the nasal sinuses.
Correct answer: Cystic fibrosis- A. Non gene transfer
- B. Limited gene transfer
- C. Excessive gene transfer
- D. High cost
Explanation: Due to limited gene transfer, this methodology has not yet been successful.
Correct answer: Limited gene transfer