Free Biotechnology MCQs with Answers

731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.

Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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731 questions · page 33 of 37

  • A. Flanking sequences
  • B. Nucleotide order
  • C. Palindromic sequences
  • D. Antagonistic sequences

Explanation: The definition and example have are given on glossary at page VIII of textbook of biology part-II.

Correct answer: Palindromic sequences
  • A. Polymerase
  • B. Helicase
  • C. Restriction endonuclease
  • D. Ligase

Explanation: The definition is on glossary page IX of textbook of biology part II.

Correct answer: Restriction endonuclease
  • A. DNA sequencing
  • B. Genetic probe
  • C. Gel electrophoresis
  • D. Polymerase chain reaction

Explanation: PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified, and then multiple…

Correct answer: Polymerase chain reaction
  • A. Cloning vectors
  • B. DNA probes
  • C. Gene targets
  • D. Restriction enzymes

Explanation: A gene bank or genomic library is a complete collection of cloned DNA fragments that comprises the entire genome of an organism.

Correct answer: DNA probes
  • A. Hypoxanthine-guanine transferase
  • B. Xanthine oxidase
  • C. PRPP synthetase
  • D. Adenosine deaminase

Explanation: The deficiency of Adenosine deaminase (ADA) causes immunodeficiency. ADA is an enzyme that is responsible for breaking down adenosine, a…

Correct answer: Adenosine deaminase
  • A. has
  • B. have
  • C. were
  • D. was

Explanation: The correct answer is has because the construction 'Not only...but also' typically treats the closest subject as the primary subject for…

Correct answer: has
  • A. Pakistan
  • B. Australia
  • C. New Zealand
  • D. West Indies

Explanation: To determine which country has lost the greatest number of times, we need to analyze the match outcomes:Pakistan defeated Australia twice…

Correct answer: New Zealand

653. A plasmid is

  • A. A chromosome
  • B. Circular extrachromosomal DNA
  • C. A protein
  • D. A virus

Explanation: Plasmids are small, circular, extrachromosomal DNA molecules in bacteria used as vectors in genetic engineering.

Correct answer: Circular extrachromosomal DNA
  • A. Natural breeding
  • B. In vitro fertilization
  • C. Somatic cell nuclear transfer
  • D. Gene therapy

Explanation: Dolly was the first mammal cloned from an adult somatic cell using nuclear transfer (SCNT).

Correct answer: Somatic cell nuclear transfer
  • A. Mutation ⎯→ Deficient ADA ⎯→ Immature T and B cells ⎯→ SCIDS
  • B. SCIDS ⎯→ Deficient ADA ⎯→ Immature T and B cells ⎯→ Mutation
  • C. Mutation ⎯→ Deficient ADA ⎯→ SCIDS ⎯→ Immature T and B cells
  • D. Mutation ⎯→ Immature B and T cells ⎯→ Deficient ADA ⎯→ SCIDS

Explanation: When that gene which is responsible for the formation of ADA mutates. ADA will be formed and as a result Tand B cells will remain unable…

Correct answer: Mutation ⎯→ Deficient ADA ⎯→ Immature T and B cells ⎯→ SCIDS
  • A. Parthenogenesis
  • B. Parthenocarpy
  • C. Cutting
  • D. Grafting

Explanation: Parthenogenesis is a form of reproduction in which an egg develops into an embryo without fertilization.

Correct answer: Cutting
  • A. Cl- ions
  • B. Na+ ions
  • C. K+ ions
  • D. Ca2+ ions

Explanation: This is the correct answer. Cystic fibrosis (CF) is a genetic disorder caused by mutations in the gene that codes for the cystic fibrosis…

Correct answer: Cl- ions
  • A. Restriction enzyme
  • B. DNA Ligase
  • C. Macrosatellites
  • D. Probes for genetic markers

Explanation: In DNA fingerprinting, probes for genetic markers are used to identify markers such as microsatellites and thus produce distinctive…

Correct answer: Probes for genetic markers
  • A. Techniques used to identify physical fingerprints of individuals
  • B. Molecular analysis of DNA profiles to identify individuals
  • C. Use of imprinting devices to analyze DNA samples
  • D. Combination of physical fingerprint identification and DNA analysis using imprinting devices

Explanation: DNA fingerprinting, also known as DNA profiling, is a molecular technique used to identify individuals based on unique patterns within…

Correct answer: Molecular analysis of DNA profiles to identify individuals
  • A. Transgenic mice for testing the safety of polio vaccine before use in humans
  • B. Transgenic models for studying new treatments for certain cardiac diseases
  • C. Transgenic cow­-Rosie which produces high fat milk for making ghee
  • D. Animals like bulls for farm work as they have extra power

Explanation: Many transgenic animals are designed to increase our understanding of how genes contribute to the development of diseases.

Correct answer: Transgenic mice for testing the safety of polio vaccine before use in humans