Free Biotechnology MCQs with Answers

731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.

Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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731 questions · page 13 of 37

  • A. Man
  • B. Virus
  • C. Bacteria
  • D. None of the above

Explanation: Bacterial cells take up recombinant plasmid,especially, if they are treated with calcium chloride to make them more permeable.

Correct answer: Bacteria
  • A. NaCl
  • B. CaCl2
  • C. Na2CO3
  • D. CaCO3

Explanation: Bacterial cells take up recombinant plasmid,especially, if they are treated with calcium chloride to make them more permeable

Correct answer: CaCl2
  • A. Clone
  • B. Probe
  • C. RFLP
  • D. Endonuclease

Explanation: A probe is a single stranded nucleotide sequence that will hybridize (pair) with a certain pieceof DNA

Correct answer: Probe
  • A. Bacteria
  • B. Bacteriophage
  • C. Segment of DNA
  • D. None of the above

Explanation: A genome is a full set of genes of an individual. A genomic library is a collection ofbacterial or bacteriophage clones, each clone…

Correct answer: None of the above
  • A. Kary Mullis
  • B. Hamilton
  • C. Sanger
  • D. Maxam

Explanation: Kary B. Mullis developed the polymerase chain reaction (PCR) in 1983.

Correct answer: Kary Mullis
  • A. Primer DNA
  • B. Recombinant DNA
  • C. DNA polymerase
  • D. Thermocycler

Explanation: Before carrying out PCR, primers - sequences of about 20 bases that are complementaryto the bases on either side of the "target DNA" -…

Correct answer: Primer DNA
  • A. Primer DNA
  • B. Recombinant DNA
  • C. DNA polymerase
  • D. Thermocycler

Explanation: DNA polymerase is a temperature-insensitive (thermostable) enzyme extractedfrom the bacterium Thermus aquaticus

Correct answer: DNA polymerase
  • A. RFLP
  • B. Probes
  • C. PCR
  • D. All of the above

Explanation: The genome is treated with restriction enzymes, which resultsin a unique collection of different-sized fragments.

Correct answer: All of the above
  • A. Probes
  • B. RFLP
  • C. PCR
  • D. Gel

Explanation: The genome is treated with restriction enzymes, which resultsin a unique collection of diferent sized fragments.

Correct answer: RFLP
  • A. Forensic
  • B. Diagnosis
  • C. Evolution
  • D. All of the above

Explanation: PCR amplification and analysis can be used (1) to diagnose viral infections, geneticdisorders, and cancer (2) in forensic laboratories to…

Correct answer: All of the above
  • A. Diagnosis
  • B. Forensic
  • C. Evolution
  • D. All of the above

Explanation: DNA analysis is used in forensic laboratories to identify criminals.The DNA from a single sperm is enough to identify a suspected rapist

Correct answer: Forensic
  • A. Deoxyribonucleotide
  • B. Ribonucleoside
  • C. Dideoxyribonucleoside
  • D. DNA threads

Explanation: For a generation of different-sized DNA fragments, two methods are generally used.

Correct answer: Dideoxyribonucleoside
  • A. Deoxyribonucleotide
  • B. Dexoyribonucleoside
  • C. Dideoxyribonucleoside
  • D. DNA threads

Explanation: For a generation of different-sized DNA fragments, two methods are generally used.

Correct answer: DNA threads
  • A. Agarol
  • B. Agarose gel
  • C. Agar
  • D. All of the above

Explanation: Pieces of DNA are separated on agarose gel

Correct answer: Agarose gel
  • A. Initiation nucleotides
  • B. Middle nucleotides
  • C. Terminating nucleotides
  • D. None of the above

Explanation: DNA sequence is now completely automated, robotic devices mix the reagents andthen load, run and read the order of the nucleotide bases…

Correct answer: Terminating nucleotides
  • A. Computer
  • B. Gel
  • C. Detector
  • D. Laser beam

Explanation: A detector positionednear the bottom of the gel reads and records the color of the fluorescent label on each bandas it passes through a…

Correct answer: Detector
  • A. Computer
  • B. Gel
  • C. Detector
  • D. Laser beam

Explanation: A computer reads and stores the nucleotidesequence.

Correct answer: Computer
  • A. Chloroplast
  • B. Mitochondria
  • C. Yeast
  • D. All of the above

Explanation: The automation of DNA sequencing, the genomes of many organisms have been sequenced.

Correct answer: All of the above
  • A. Yeast
  • B. Pea
  • C. Arabidopsis
  • D. Plum

Explanation: Arabidopsis is model plant whose gene sequencing has been completed

Correct answer: Arabidopsis
  • A. 12
  • B. 18
  • C. 21
  • D. 22

Explanation: The DNA sequence of human chromosome no. 22, one of the smallest human chromosomes, was completed in 1999

Correct answer: 22