The steps involved in DNA finger printing:
Correct answer: D. All of the above
- A. RFLP
- B. Probes
- C. PCR
- D. All of the above
Explanation
The genome is treated with restriction enzymes, which resultsin a unique collection of different-sized fragments. Therefore, restriction fragmentlength polymorphism (RFLPs) exists between individuals. The use of probes for genetic markers produces adistinctive pattern that can be recorded on X-ray film. PCR amplification and analysis can be used (1) to diagnose viral infections, geneticdisorders, and cancer (2) in forensic laboratories to identify criminals; and (3) todetermine the evolutionary history of the human population.
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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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