Free Biotechnology MCQs with Answers
731 Biotechnology MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.
Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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- A. Insulin
- B. Drugs
- C. Pollution
- D. All of the above
Explanation: Modern techniques enable desired substances, for example, insulin. Not very long ago, people with insulin-dependentthey receive human…
Correct answer: All of the above- A. Replication
- B. Cloning
- C. Reproduction
- D. Multiplication
Explanation: The cloning of a gene produces many identical copies.
Correct answer: Cloning- A. PCR
- B. Recombinant DNA technology
- C. RFLP
- D. None of the above
Explanation: Cloning of a gene produces many identical copiesPolymerase chain reaction (PCR) creates a lesser number of copies within a laboratory test…
Correct answer: None of the above- A. PCR
- B. Recombinant DNA technology
- C. RFLPs
- D. None of the above
Explanation: The use of polymerase chain reaction (PCR) creates a lesser number of copies within a laboratory test tube.
Correct answer: PCR- A. Molecular scissors
- B. Molecular vector
- C. Expression system
- D. None of the above
Explanation: A vector, as related to molecular biology, is a DNA molecule (often plasmid or virus) that is used as a vehicle to carry a particular DNA…
Correct answer: Molecular vector- A. Molecular scissors
- B. Molecular vector
- C. Expression system
- D. Gene of interest
Explanation: The gene of interest along with the vector is then introduced into an expressionsystem, as a result of which a specific product is made.
Correct answer: Expression system227. Genes are cut by:
- A. Gene of interest
- B. Expression system
- C. Molecular vector
- D. Endonucleases
Explanation: Genes can be isolated from the chromosomes by cutting the chromosomes on theflanking sites of the gene using special enzymes known as…
Correct answer: Endonucleases- A. Endonucleases
- B. Plasmids
- C. cDNA
- D. None of the above
Explanation: This involves converting the RNA back into a DNA copy, known as complementary DNA (cDNA) by reverse transcriptase.
Correct answer: cDNA- A. Virus
- B. Bacteria
- C. Plasmids
- D. All of the above
Explanation: These are natural enzymes of bacteria, which they use for their protection againstviruses.
Correct answer: Bacteria- A. Kary Mullis
- B. Hamilton
- C. Sanger
- D. Maxam
Explanation: In 1970, Hamilton O. Smith, at Johns Hopkins University, isolated thefirst restriction enzyme.
Correct answer: Hamilton- A. Single phase
- B. Repeated form
- C. Reverse order
- D. Similar order
Explanation: Bacteria produce a variety of restriction enzymes, whichcut the DNA at very specific sites characterized by a specific sequence of four or…
Correct answer: Reverse order- A. 200
- B. 300
- C. 400
- D. 500
Explanation: More than 400 restriction enzymes have been isolated.
Correct answer: 400- A. 10
- B. 20
- C. 30
- D. 40
Explanation: More than 400 restriction enzymes have been isolated outof which about 20 are frequently used in recombinant DNA technology.
Correct answer: 20- A. E.Coli
- B. EcoR11
- C. EcoR1
- D. EcoR111
Explanation: EcoRl, a commonly used restriction enzyme, cuts double-stranded DNA.
Correct answer: EcoR1- A. Endonucleases
- B. Restriction enzyme
- C. Plasmids
- D. Phage virus
Explanation: Plasmids are natural extra-chromosomal circular DNA molecules which carry genes forantibiotic resistance and fertility.
Correct answer: Plasmids- A. pSc 101
- B. pBR-322
- C. EcoR1
- D. Phage virus
Explanation: EcoRl, a commonly used restriction enzyme, cuts double-stranded. It is not a vector.
Correct answer: EcoR1- A. pSc 101
- B. pBR-322
- C. EcoR1
- D. Phage virus
Explanation: pBR 322 has antibiotic resistancegenes for tetracycline as well as ampicillin.
Correct answer: pBR-322- A. Bacteriophage
- B. Lambda phage
- C. T phage
- D. All of the above
Explanation: Besides plasmids, the DNA of bacterial viruses (for example, lambda phage) can also be usedas a vector.
Correct answer: Lambda phage- A. Chimaeric DNA
- B. DNA ligase
- C. Plasmids
- D. Endonucleases
Explanation: DNA ligase enzyme seals the foreign piece ofDNA into the vector.
Correct answer: DNA ligase- A. Chimaeric DNA
- B. DNA ligase
- C. Plasmids
- D. Endonucleases
Explanation: Recombinant DNA is also called chimaeric DNA.
Correct answer: Chimaeric DNA