Two bacteria found to be very useful in genetic engineering experiments are?
Correct answer: D. Escherichia and Agrobacterium
- A. Nitrobacter and Azotobacter
- B. Rhizobium and Diplococcus
- C. Nitrosomonas and Kliebsiella
- D. Escherichia and Agrobacterium
Explanation
E.coli contains many important standard cloning vectors widely used in gene cloning experiments like pBR322 contains origin of replication (ori). Other cloning vectors like PACYC177, pBR324, PRK 64.6 contain ampicillin resistance gene and they are also found in E.coli. Among higher plants, Ti plasmid of Agrobacterium tumefaciens and Ri plasmid of A. rhizogenes is the best known vector. T-DNA from Ti or Ri plasmid of Agrobacterium is considered to be a very potential vector for cloning experiments with higher plants.
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