Plasmid has been used as a vector because?
Correct answer: A. It is circular DNA which have capacity to join to eukaryotic DNA.
- A. It is circular DNA which have capacity to join to eukaryotic DNA.
- B. It can move between prokaryotic and eukaryotic cells.
- C. Both ends show replication.
- D. It has antibiotic resistance gene.
Explanation
Plasmid and bacteriophage are used as vectors in genetic engineering. Plasmid is an autonomously replicating circular extra chromosomal DNA found in bacteria. They can be transferred from cell to cell in a bacterial colony. This characteristic is being used in biotechnology for transferring desirable gene into the target gene of the host. Bacteriophage is a bacterial virus that can infect it, quickly multiply within and destroy (lyse) their host (virus) cells. During infection, bacteriophages inject their DNA into these cells. The injected DNA selectively replicate and are expressed in the host that results in a multiplication of phages that ultimately burst out of the cell (by lysis). This ability to transfer DNA from the phage genome to a specific host during the infection process gave scientists the idea that specially designed phage vectors could be used for gene cloning.
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