In Lederberg's replica plating experiment what shall be used to obtain streptomycin resistant strain?
Correct answer: A. Minimal medium and Streptomycin
- A. Minimal medium and Streptomycin
- B. Complete medium and Streptomycin
- C. Only minimal medium
- D. Only complete medium.
Explanation
If Streptomycin resistant mutant is to be obtained, the material should be allowed to grow on medium lacking streptomycin so that both mutant and wild types may grow. These colonies are imprinted on Petri plates to form the master pattern and other plates having streptomycin can then be pressed on velveteen to get an impression. The plate now containing only mutants for Streptomycin resistance will grow.
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