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Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

Correct answer: D. Agrobacterium tumefaciens

  • A. Clostridium septicum
  • B. Xanthomonas citri
  • C. Bacillus coagulens
  • D. Agrobacterium tumefaciens

Explanation

Agrobacterium tumefaciens has been extensively used in genetic engineering experiments. It is the causative agent of crown gall, an important disease of many commercial crops. This disease has come to be recognized in recent years as being caused by a DNA plasmid (Ti plasmid) carried by bacterium and transferred to the plant cells. Following the discovery of the relationship between crown gall and the Ti plasmid, this plasmid has come to be widely used in plant genetic engineering as a vector in order to inject a novel gene in a host plant to form a transgenic plant.

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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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