Genetic engineering is possible, because?
Correct answer: B. Restriction endonucleases purified from bacteria can be used in vitro.
- A. We can cut DNA at specific sites by endonucleases like DNase I.
- B. Restriction endonucleases purified from bacteria can be used in vitro.
- C. The phenomenon of transduction in bacteria is well understood.
- D. We can see DNA by electron microscope.
Explanation
DNA restriction endonuclease are an important class of restriction exonucleases, class II, which cut doublestranded DNA molecules only at sites characterized by a specific nucleotide sequence. Restriction enzymes are isolated from bacterial cells, and are tools for molecular biologists. Several hundred restriction enzymes are now known, each with a specific sequence requirement dictating where it will cut DNA. Some, such as Hind III, make staggered cuts leaving 'sticky ends', three nucleotides long protruding on one strand from each severed terminus; others make clean cuts in both strands at the same place and thus generate 'blunt ends'. Digesting DNA with a restriction enzyme therefore creates a characteristic set of fragments, which can be isolated by electrophoresis and subsequently analyzed.
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