Restriction endonucleases are:
Correct answer: B. Used in genetic engineering.
- A. Used for in vitro DNA synthesis.
- B. Used in genetic engineering.
- C. Synthesized by bacteria.
- D. Present in mammalian cells for degradation of DNA.
Explanation
Restriction endonucleases were found by Arber in 1962 in bacteria. They act as "molecular scissors" or chemical scalpels. They recognize the specific base sequence at palindrome sites in DNA duplex and cut its strands. For example, restriction endonuclease EcoRI found in the colon bacteria E.coli reocgnizes the base sequence GAATTC in DNA duplex and cuts its strands between G and A.
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