Moderate

The DNA molecule to which the gene of interest is integrated for cloning is called:

Correct answer: D. Vector

  • A. Template
  • B. Carrier
  • C. Transformer
  • D. Vector

Explanation

Vector is a DNA molecule that carries a foreign DNA segment and replicates inside a host cell. The vector DNA and foreign DNA carrying gene of interest are cut by the same restriction endonuclease enzyme to produce complementary sticky ends. With the help of DNA ligase enzyme, the complementary sticky ends of the two DNAs are joined to produce a recombinant DNA (rDNA), which is then introduced into the host cell.

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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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