Which of the following is a restriction endonuclease?
Correct answer: A. DNAase I
- A. DNAase I
- B. RNAase
- C. Hind II
- D. Protease
Explanation
Hind II is the first restriction endonuclease. It was isolated from Haemophilus influenzae Rd. It always cut DNA at a specific position producing blunt ends. DNase I is an endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide nonspecially. RNase is a type of nuclease that catalyses the degradation of RNA into smaller components. It can be endoribonuclease or exoribonuclease. A protease is an enzyme that performs proteolysis, i.e., protein catabolism by hydrolysis of the peptide bonds.
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