In the process of centrifugation, the cell extract is subjected to centrifugal force which causesthe separation of extract into two fractions: a pellet and a supernatant.The option that identifies the materials in the pellet and supernatant is
Correct answer: A. Pellet: Nuclei, Supernatant: Dissolved ions
- A. Pellet: Nuclei, Supernatant: Dissolved ions
- B. Pellet: Dissolved ions, Supernatant: Dissolved ions
- C. Pellet: Nuclei, Supernatant: Nuclei
- D. Pellet: Dissolved ions, Supernatant: Nuclei
Explanation
During centrifugation, heavier and more dense components like Nuclei are forced to the bottom of the tube to form a Pellet. Lighter and less dense components, such as dissolved ions, remain above in the supernatant. Therefore, the correct answer is that the pellet contains nuclei and the supernatant contains dissolved ions. The other options are incorrect because they either suggest that the same type of material is in both fractions or misplace the nuclei and dissolved ions.
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About Cytoplasmic Organelles
Cytoplasmic organelles are the specialized structures suspended in cytoplasm, including mitochondria, ribosomes, endoplasmic reticulum, Golgi apparatus, lysosomes, vacuoles and centrosomes. Questions compare their structures and functions in animal and plant cells, including chloroplasts and the role of the endomembrane system in transport and secretion.
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