DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by?
Correct answer: A. Electrophoresis
- A. Electrophoresis
- B. Restriction mapping
- C. Centrifugation
- D. Polymerase chain reaction
Explanation
Electrophoresis is a technique used for the separation of substances of different ionic properties. Since the DNA fragments are negatively charged molecules, they can be separated by allowing them to move towards the anode. DNA fragments move towards the anode according to the size of their molecules through the pores of agarose gel. Thus, the smaller fragments move farther away as compared to larger fragments.
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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
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