During the process of isolation of DNA, chilled ethanol is added to
Correct answer: A. Precipitate DNA.
- A. Precipitate DNA.
- B. Break open the cell to release DNA.
- C. Facilitate action of restriction enzymes.
- D. Remove proteins such as histones.
Explanation
Ethanol is much less polar than water. Adding it to the solution disrupts the screening charges exerted by water. The electrical attraction between phosphate and any positive ions (Na+) present in the solution becomes strong enough to form a stable ionic bond and DNA precipitates. Ethanol precipitation is a widely used technique to purify or concentrate nucleic acid.
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