Moderate

PCR and restriction fragment length polymorphism(RFLP)are the methods for?

Correct answer: D. Genetic fingerprinting

  • A. Study of enzymes
  • B. Genetic transformation
  • C. DNA sequencing
  • D. Genetic fingerprinting

Explanation

Polymerase chain reaction (PCR) is used to amplify a small DNA fragment to obtain its large quantity. PCR is very helpful in DNA fingerprinting in such cases where the culprit has to be identified from a very small blood, semen or another cell sample from a crime scene. Restriction fragment length polymorphism(RFLP) is used to determine or confirm the source of DNA sample such as in paretnity tests or criminal investigtaions In genetic mapping to determine recombination rates that show the genetic distance between the loci. To identify a carrier of a disease-causing mutation in a family.

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Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.

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