Commonly used vectors for human genome sequencing are:
Correct answer: B. Bacterial artificial chromosome & Yeast artificial chromosome
- A. T-DNA
- B. Bacterial artificial chromosome & Yeast artificial chromosome
- C. Expression vectors
- D. TA cloning vectors
Explanation
Bacterial artificial chromosome (BAC) vectors are based on the natural, extra-chromosomal plasmid of E. coli. BAC vector contains genes for replication and maintenance of the F-factor, a selectable marker and cloning site. These vectors can accommodate up to 300-350 kb(kilo base) of foreign DNA and are also being used in genome sequencing project. Yeast artificial chromosome (YAC) vectors are used to clone DNA fragments of more than 1Mb in size. Therefore, they have been exploited extensively in mapping the large genomes, e.g., in the Human Genome Project. These vectors contain the telomeric sequence, the centromere and the autonomously replicating sequence from yeast chromosomes.
Last updated
About Biotechnology
Biotechnology in health care applies biological organisms, cells or enzymes to diagnose and treat disease. The subject includes recombinant DNA, genetically engineered insulin, vaccines, antibiotics, monoclonal antibodies, gene therapy and techniques such as polymerase chain reaction, while distinguishing therapeutic applications from agricultural and industrial biotechnology.
Practise Biotechnology
731 free Biotechnology MCQs from Biology, each with the correct answer and an explanation. Unlimited attempts, no account needed.
Exams that ask Biology questions like this
Biology is on 6 papers prepared for on TestUstad, and all of them draw the same bank, so this question is worth knowing for every one of them.
Related questions
A clone can be identical copy of a/an:
A collection of plants and seeds having diverse alleles of all the genes of a crop is called
A disadvantage of animal cloning is:
A DNA molecule synthesized from mRNA by reverse transcriptase in laboratory is called?
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using: