Free Factors Affecting Enzyme Action MCQs with Answers

40 Factors Affecting Enzyme Action MCQs from Biology, each with the correct answer and a written explanation of why it is correct. Free and unlimited, with no account needed.

Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.

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40 questions · page 2 of 2

  • A. At point I, hydrogen bonds are formed between enzyme and substrate
  • B. At point II, kinetic energy of enzyme and substrate is the highest.
  • C. At point III, peptide bonds in the enzyme begin to break
  • D. At point IV, the enzyme is completely denatured

Explanation: The correct answer is Option A. At point I, the enzyme operates under optimal conditions, allowing for effective formation of…

Correct answer: At point I, hydrogen bonds are formed between enzyme and substrate
  • A. X and Y the number of enzyme molecules is limiting the rate of reaction
  • B. X and Y the number of product molecules is limiting the rate of reaction
  • C. Y and Z the number of enzyme molecules is limiting the rate of reaction
  • D. Y and Z the number of substrate molecules is limiting the rate of reaction

Explanation: Between X and Y, increasing the substrate concentration increases the rate of reaction because all active sites of enzymes are not…

Correct answer: Y and Z the number of enzyme molecules is limiting the rate of reaction
  • A. Point I only
  • B. Point III only
  • C. Points I and II
  • D. Points II and III

Explanation: The rate of an enzyme-catalyzed reaction increases with substrate concentration until all enzyme active sites are occupied, at which point…

Correct answer: Points II and III
  • A. A
  • B. B
  • C. C
  • D. D

Explanation: The temperature at which enzyme shows maximum activity is called optimum temperature.Enzyme activity increases up to optimum temperature.

Correct answer: D
  • A. I
  • B. II
  • C. III
  • D. IV

Explanation: The temperature at which enzyme shows maximum activity is called optimum temperature.Enzyme activity increases up to optimum temperature.

Correct answer: IV
  • A. Enzymes start to denature
  • B. Enzymes start to re-nature
  • C. Enzymes work best
  • D. Enzymes are reactivated

Explanation: Maximum temperature refers to a temperature at which enzymes start to breakdown and denature.

Correct answer: Enzymes start to denature
  • A. Saturation
  • B. Inhibition
  • C. Denaturation
  • D. Composition

Explanation: Enzymes are protein in nature. At a temperature higher than optimum temperature, the 3D shape of active site denatures and substrate…

Correct answer: Denaturation
  • A. Enzyme start denaturing
  • B. Enzyme becomes hyperactive
  • C. Enzyme work at their best
  • D. Inactive enzyme getting reactived

Explanation: Minimum temperature is the term used when an inactive enzyme starts getting reactive.

Correct answer: Inactive enzyme getting reactived
  • A. 30oC
  • B. 40oC
  • C. 50oC
  • D. 20oC

Explanation: Enzymes generally have an optimal temperature at which they exhibit their highest activity.

Correct answer: 40oC
  • A. Enzymes start to denature
  • B. Enzymes start to re-nature
  • C. Enzymes work best
  • D. Enzymes are reactivated

Explanation: The term "maximum temperature" means the point at which enzymes start to denature (Option A).

Correct answer: Enzymes start to denature
  • A. Maximum temperature
  • B. Absolute temperature
  • C. Critical temperature
  • D. Optimum temperature

Explanation: Enzymes are biological catalyst, which are used to speed up the rate of chemical reactions taking place in body.

Correct answer: Optimum temperature
  • A. It increases the enzyme activity
  • B. Stops the reaction
  • C. Decreases the enzyme activity
  • D. Both Options B and C are correct

Explanation: is temperature of a reaction medium increases above the optimum temperature then it stops reaction by decreasing enzyme activity because…

Correct answer: Both Options B and C are correct
  • A. Denaturation
  • B. Composition
  • C. Inhibition
  • D. Saturation

Explanation: Saturation refers to the point in an enzymatic reaction where all of the enzyme's active sites are occupied by substrate molecules…

Correct answer: Saturation
  • A. 3.00
  • B. 4.50
  • C. 2.00
  • D. 6.00

Explanation: Pepsin works best at a pH of around 2.00, which is highly acidic. The low pH helps denature proteins and allows pepsin to cleave peptide…

Correct answer: 2.00
  • A. Specific Temperature
  • B. Specific pH
  • C. Specific Co-enzyme
  • D. All the above

Explanation: The correct answer is 'All the above' because enzymes require specific conditions to function at their best.

Correct answer: All the above
  • A. 4.5
  • B. 5.5
  • C. 6.1 - 6.8
  • D. 6.7 - 7

Explanation: Maltase is an enzyme that catalyzes the hydrolysis of the disaccharide maltose to the simple sugar glucose.

Correct answer: 6.1 - 6.8
  • A. Substrate concentration
  • B. pH
  • C. Enzyme concentration
  • D. Temperaturep

Explanation: Substrate concentration refers to the amount of substrate present in a chemical reaction.

Correct answer: Substrate concentration
  • A. 3
  • B. 3.5
  • C. 2
  • D. 2.5

Explanation: The stomach contains gastric glands that secrete hydrochloric acid (HCl) along with pepsinogen, an inactive precursor of pepsin.

Correct answer: 2