Asked in UHS MDCAT 2015 2015Moderate

Pepsin, protein digesting enzymes, sets best pH:

Correct answer: C. 2.00

  • A. 3.00
  • B. 4.50
  • C. 2.00
  • D. 6.00

Explanation

Pepsin works best at a pH of around 2.00, which is highly acidic. The low pH helps denature proteins and allows pepsin to cleave peptide bonds efficiently.

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About Factors Affecting Enzyme Action

Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.

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