The enzyme used to seal the gene permanently into the plasmid is
Correct answer: C. DNA ligase
- A. restriction endonuclease
- B. reverse transcriptase
- C. DNA ligase
- D. helicase
Explanation
Sticky ends pair by hydrogen bonds only, which is not strong enough to hold, so ligase forms the covalent phosphodiester bonds in the sugar phosphate backbone and completes the recombinant molecule. Restriction enzymes do the opposite job of cutting, which is why the two are always taught as a pair. Ligase is the same enzyme that joins Okazaki fragments during normal DNA replication.
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Biotechnology applies genetic engineering, cell culture and recombinant DNA methods to medicine and public health. The material includes production of insulin, vaccines and antibiotics, diagnostic techniques, gene therapy, genetically modified organisms and the ethical concerns surrounding manipulation of human genes.
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