Sequence of amino acids in insulin molecules were determined by:

Explanation

The sequence of amino acids in insulin molecules was determined by Frederick Sanger in the early 1950s. Sanger developed the first method for sequencing proteins, which he used to determine the precise sequence of amino acids in insulin. His groundbreaking work earned him the Nobel Prize in Chemistry in 1958. Sanger's method, known as the Sanger sequencing method or chain-termination method, revolutionized the field of molecular biology and laid the foundation for many subsequent advances in genetics and protein biochemistry.