Okazaki fragments are connected together through action of_________________?
Correct answer: D. Ligase
- A. Primase
- B. Polymerase
- C. Helicase
- D. Ligase
Explanation
DNA ligase joins Okazaki fragments on the lagging strand by sealing breaks in the sugar-phosphate backbone and forming phosphodiester bonds. Primase makes RNA primers, polymerase adds nucleotides, and helicase separates the DNA strands. Students often choose polymerase because it synthesises DNA, but it does not join the completed fragments together.
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About Structure of DNA
DNA structure includes nucleotides made of deoxyribose, phosphate and nitrogenous bases, arranged in two antiparallel polynucleotide strands. Questions cover the double helix, phosphodiester and hydrogen bonds, complementary base pairing between adenine and thymine and between guanine and cytosine, and the significance of major and minor grooves.
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