Asked in Premeth past paper pool — BiologyModerate

In genetic engineering, DNA probes are used:

Correct answer: D. Search for a certain gene in a genetic library

  • A. Clone a DNA fragment
  • B. Isolate a gene from the bacterium
  • C. Detect the presence of mutation in a gene
  • D. Search for a certain gene in a genetic library

Explanation

The correct answer is Option D: DNA probes are used to search for a certain gene in a genetic library. DNA probes are short, labeled, single-stranded sequences of DNA that hybridize to complementary sequences in the genome, allowing researchers to identify the presence of specific genes. Option A is incorrect because cloning involves replication rather than identification. Option B is not correct as isolation requires different techniques, though probes can aid in identification. Option C, while true in other contexts, is not the primary use of probes in genetic libraries.

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Biotechnology applies genetic engineering, cell culture and recombinant DNA methods to medicine and public health. The material includes production of insulin, vaccines and antibiotics, diagnostic techniques, gene therapy, genetically modified organisms and the ethical concerns surrounding manipulation of human genes.

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