If the concentration of enzyme is kept constant and amount of substrate is increased a point is reached where increase in substrates concentration does not affect the reaction rate because of____________________?
Correct answer: C. All the active sites on enzyme molecule are occupied
- A. Enzymes get denatured at higher substrate conc
- B. Rate of reaction is indirectly proportional to substrate concentration at this point
- C. All the active sites on enzyme molecule are occupied
- D. None of these
Explanation
With a fixed enzyme concentration, increasing substrate eventually occupies all available active sites, so the enzymes are working at their maximum rate. The likely mistake is choosing a, but high substrate concentration does not normally denature enzymes; it produces saturation instead.
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About Factors Affecting Enzyme Action
Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.
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