If enzyme concentration is low than substrate pH and temperature values are equal to requirement then which of the following will increase rate of reaction____________________?
Correct answer: A. increase in concentration of enzyme
- A. increase in concentration of enzyme
- B. increase in concentration of substrate
- C. increase in pH
- D. increase in temperature
Explanation
When pH and temperature are already optimal and enzyme concentration is limiting, adding more enzyme provides more active sites and increases the reaction rate. Increasing substrate cannot solve the shortage of enzyme once the available active sites are being used. The likely mistake is choosing b by assuming that more substrate always produces a faster reaction.
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About Factors Affecting Enzyme Action
Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.
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