Amylase becomes denatured at an alkaline pH of:__________?
Correct answer: D. 9
- A. 6
- B. 7
- C. 8
- D. 9
Explanation
Salivary amylase is suited to the nearly neutral pH of the mouth and loses its functional shape in strongly alkaline conditions, such as around pH 9. Students may choose pH 8 because it is also alkaline, but pH 9 is the standard textbook value given for denaturation of salivary amylase.
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About Factors Affecting Enzyme Action
Enzyme activity changes with temperature, pH, substrate concentration and enzyme concentration because these factors affect molecular collisions and the active site. Questions also examine optimum conditions, denaturation and inhibition, while distinguishing a fall in activity caused by unsuitable conditions from permanent enzyme damage.
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